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Document
Published on
2022 May 12Burundi Agricultural Research Bulletin No.
Two species of taro are cultivated in Burundi: Colocasia esculenta and Xanthosoma sagittifolium. These species have become popular and contribute to the diet as the main source of energy. However, over the last 20 years, a very strong downward trend in production for both species due to biotic constraints has been observed. Preliminary field observations as well as laboratory tests have shown the prevalence of viral infections and fungi. A search conducted in 2013 at the BeCailri Hub identified the Dasheen Mosaic Virus (DsMV) on samples collected in Burundi as well as a badnavirus that was not characterized at that time. Since the fight against viruses is preventive in nature, it is necessary to obtain a reliable and relatively inexpensive detection method. Diagnostic tools that have been developed for viruses include ELISA tests and PCR tests. PCR tests are reliable, but require expensive equipment and qualified personnel, which are not always available at most research stations. The LAMP test has been identified as a reliable and simple laboratory method for the detection of viruses and other pathogens. This test works under isothermal conditions with comparable and even more specific effectiveness than PCR. The objectives of this research were as follows: - identify and validate the prevalence and distribution of viruses infecting colocases in Burundi; - develop sensitive and low-cost diagnostic tests for colocase viruses; - acquire practical experience on genomic analysis and the techniques used in the development of molecular diagnostic tests.